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目的:探讨单羧酸转运蛋白1(monocarboxylate transporters 1,MCT1)、CD147、CD44 mRNA和蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)中表达的相关性及其在NSCLC发生、发展及预后中的意义。方法 :取经病理确诊的56例NSCLC患者癌组织、癌旁组织(距肿瘤边缘>5 cm)和21例肺良性病变组织采用免疫组化和实时荧光定量PCR检测MCT1、CD147、CD44蛋白及mRNA的表达。结果:①在NSCLC组织中MCT1、CD147、CD44表达均明显高于对照组(P<0.05);②MCT1在肺癌细胞膜上的表达水平与CD147、CD44的表达均存在相关性(P<0.05);③MCT1、CD147、CD44的表达与NSCLC患者的性别、年龄、肿瘤大小、TNM分期均无关,但与肿瘤的组织类型(P<0.05)、淋巴转移(P<0.05)显著相关,且MCT1、CD44蛋白和mRNA的表达与肿瘤的分化程度显著相关(P<0.05)。结论:MCT1、CD147、CD44可能参与肿瘤的发生、发展及远处淋巴结转移,可作为检测NSCLC和预后评价的指标。
OBJECTIVE: To investigate the correlation between the expression of monocarboxylate transporters 1 (MCT1), CD147 and CD44 mRNA and protein in non-small cell lung cancer (NSCLC) and their occurrence and development in NSCLC And the significance of prognosis. Methods: The expressions of MCT1, CD147 and CD44 protein and mRNA were detected by immunohistochemistry and real-time fluorescence quantitative PCR in 56 cases of NSCLC patients with pathological diagnosis, adjacent tissues (> 5 cm from tumor margin) and 21 cases of benign lung lesions expression. Results: ① The expressions of MCT1, CD147 and CD44 in NSCLC tissues were significantly higher than those in the control group (P <0.05); ② The expression of MCT1 in lung cancer cell membrane correlated with the expression of CD147 and CD44 (P <0.05); ③ MCT1 , CD147 and CD44 expression were not related with gender, age, tumor size and TNM stage in patients with NSCLC, but significantly correlated with tumor type (P <0.05) and lymphatic metastasis (P <0.05) The mRNA expression was significantly correlated with tumor differentiation (P <0.05). Conclusion: MCT1, CD147 and CD44 may play an important role in tumorigenesis, progression and distant lymph node metastasis, which can be used as an index for the detection of NSCLC and prognosis.