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采用 HPLC法测定沙棘果油中α-生育酚的含量。C18色谱柱 ,以乙腈 -甲醇 (38∶ 62 )为流动相 ,柱温30℃ ,检测波长为 2 80 nm。结果三批沙棘果油中α-生育酚的平均含量为 0 .0 2 78% ,方法平均回收率为97.4 4 2 % (RSD =0 .95 % )。
Determination of α-tocopherol in Seabuckthorn Oil by HPLC. C18 column with acetonitrile-methanol (38:62) as the mobile phase, the column temperature was 30 ℃, the detection wavelength was 280 nm. Results The average contents of α-tocopherol in three batches of seabuckthorn oil were 0.02278%. The average recoveries were 97.442% (RSD = 0.95%).