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以火炬松×洪都拉斯加勒比松F1代群体为研究对象,从松树PGI(松类基因索引)数据中筛选出13个与赤霉素(GAs)代谢有关的序列。设计了这13条序列的EST-SSR引物对,并筛选出4对引物作为F1代检测的较好的标记。4对引物PCR分析显示在2个亲本和39个子代中共扩增出1014个多态性位点,其中,杂种F1代扩增出的位点数中有50.19%与父本相同,52.17%与母本相同,这表明母本(火炬松)和父本(加勒比松)杂交能够得到获得双亲遗传物质的新杂种。4个引物检测的26个等位基因位点中有6个与苗龄6个月的苗高有显著或极显著的相关性,有5个位点与苗龄9个月的苗高有显著或极显著相关性。这为早期选择提供了较好的分子标记。
Thirteen F1 isolates related to gibberellin (GAs) were screened from pine PGI (Pinus massoniana) F1 population using the F1 generation of Pinus taeda × Honduras. The 13 EST-SSR primer pairs were designed and 4 pairs of primers were selected as the better markers for F1 generation. Four pairs of primers showed that 1014 polymorphic loci were amplified in 2 parents and 39 progenies, among which, 50.19% of F1 loci amplified were the same as their parents, 52.17% This is the same, suggesting that the crossing of the female parent (Pinus taeda) with the male parent (Caribbean pine) can result in new hybrids that acquire the parental genetic material. Among the 26 alleles detected by 4 primers, 6 were significantly or very significantly correlated with the seedling height of 6 months of seedling age, and there were significant differences between 5 sites and seedling height of 9 months Or very significant correlation. This provided better molecular markers for early selection.