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目的观察川芎嗪注射液对急性脊髓损伤后脊髓组织中丙二醛(MDA)和超氧化物歧化酶(SOD)水平的影响。方法选用Wistar健康大鼠,参照Freeman-Wright改良WD法复制大鼠急性脊髓损伤模型,随机分为模型组(n=30)、中药组(n=30)和西药组(n=30),另设正常对照组(n=15);造模后30 min,中药组给予川芎嗪注射液200 mg·kg~(-1),西药组给予甲强龙注射液30 mg·kg~(-1),0.5 h后,各实验组于2 h、6 h、24 h、3 d、7 d时间点采用硫代巴比妥酸(TBA)比色法检测脊髓组织中的MDA、SOD的含量。结果与正常对照组比较,模型组在2 h、6 h、24 h时间点,MDA水平显著升高而SOD活性显著降低,差异有统计学意义(P<0.05);与模型组比较,中药组、西药组在各时间点的MDA水平显著降低而SOD活性显著提高,差异有统计学意义(P<0.05)。结论川芎嗪注射液能提高脊髓组织中SOD活性,并抑制MDA的生成,有明显的抗自由基损伤能力,对大鼠急性脊髓损伤早期具有保护作用。
Objective To observe the effects of ligustrazine injection on the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the spinal cord after acute spinal cord injury. Methods Wistar rats were randomly divided into model group (n = 30), Chinese medicine group (n = 30) and western medicine group (n = 30) according to Freeman-Wright modified WD method. The normal control group (n = 15) was given 30 mg · kg ~ (-1) tetramethylpyrazine injection 200 mg · kg ~ 0.5 h later, the contents of MDA and SOD in spinal cord tissue were measured by thiobarbituric acid (TBA) colorimetric method at 2 h, 6 h, 24 h, 3 d and 7 d respectively. Results Compared with the normal control group, the MDA level increased significantly and the SOD activity decreased significantly in the model group at 2 h, 6 h and 24 h (P <0.05). Compared with the model group, , The western medicine group at each time point significantly reduced the level of MDA and SOD activity was significantly increased, the difference was statistically significant (P <0.05). Conclusion Ligustrazine injection can increase the activity of SOD in spinal cord tissue and inhibit the formation of MDA. It has obvious anti-free radical damage ability and has a protective effect on the early stage of acute spinal cord injury in rats.