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目的:优选丹参-人参活性组分抗肝癌优化配伍并对其作用机制作初步研究。方法:以人肝癌细胞SMMC-7721为研究对象,人正常肝细胞L-O2为对照,采用CCK-8法以对肝癌细胞增殖抑制和正常肝细胞保护作用为筛选指标,正交设计优选丹参-人参活性组分抗肝癌有效组合,确定优化配伍;应用实时细胞分析技术(RTCA DP)检测丹参-人参组分优化配伍对肝癌细胞SMMC-7721和正常肝细胞L-O2增殖的影响,并进行时效关系考察;采用Annexin V/PI双染法经高内涵细胞成像系统(HCS)检测丹参-人参组分优化配伍对肝癌细胞SMMC-7721凋亡的影响。结果:丹参-人参组分配伍抗肝癌SMMC-7721细胞的优化组合为丹参总酚酸、人参总皂苷和人参多糖,其配伍剂量分别为10,10,5 mg·L-1;优选的丹参-人参组分配伍能显著抑制肝癌细胞的增殖,呈现时间依赖性,而对正常肝细胞的增殖抑制作用不明显;丹参-人参组分优化配伍能诱导肝癌细胞凋亡,对正常肝细胞的凋亡无显著影响。结论:丹参-人参组分优化配伍具有潜在的特异选择性抗肝癌作用,其机制与抑制肝癌细胞增殖和诱导细胞凋亡相关。
Objective: To optimize the active ingredient of Salvia - ginseng anti-liver cancer optimal compatibility and its mechanism of action for a preliminary study. Methods: Human hepatocellular carcinoma cell SMMC-7721 was used as the research object. Human normal hepatocyte L-O2 was used as a control. CCK-8 method was used to screen the hepatoma cell proliferation inhibition and normal hepatocyte protective effect. The active components of ginseng against liver cancer effective combination to determine the optimal compatibility; application of real-time cell analysis (RTCA DP) to detect Salvia - ginseng components of the optimal combination of hepatoma cells SMMC-7721 and normal liver cells L-O2 proliferation, and aging The relationship between Danshen-Ginseng (Ginseng) extract and apoptosis of hepatocellular carcinoma cell line SMMC-7721 was detected by Annexin V / PI double staining and high content cell imaging system (HCS). Results: The optimal combination of Salvia miltiorrhiza-ginseng and anti-hepatoma SMMC-7721 cells was Salvia miltiorrhiza total phenolic acid, total ginsenoside and ginseng polysaccharide with the compatibility doses of 10, 10 and 5 mg · L-1, respectively. The compatibility of ginseng components can significantly inhibit the proliferation of hepatoma cells in a time-dependent manner, while the inhibitory effect on the proliferation of normal hepatocytes is not obvious. The optimal combination of radix salviae miltiorrhizae and ginseng components can induce the apoptosis of hepatoma cells and the apoptosis of normal hepatocytes No significant effect. Conclusion: Danshen - ginseng has the potential of selective and selective anti - hepatocarcinoma, and its mechanism is related to inhibiting proliferation and inducing apoptosis of hepatocellular carcinoma cells.