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目的:以喉鳞状细胞癌为研究对象,探讨FHIT基因蛋白表达在喉癌发生、发展中的作用及临床意义。方法:用免疫组织化学SP法检测52例喉癌组织、23例癌旁组织及10例正常组织中FHIT基因蛋白的表达,分析在不同临床病理指标下FHIT蛋白表达的情况。结果:FHIT蛋白在正常组织中的阳性表达率(90.0%)及癌旁组织中的阳性率(78.3%)均高于癌组织中的阳性率(46.2%),差异有统计学意义(均P<0.01)。癌组织FHIT蛋白阳性表达率与临床分期和颈淋巴结转移有关,与病理分级无关。结论:喉癌的发生、发展与FHIT抑癌基因的失活有密切关系.FHIT基因很可能是喉癌重要的侯选抑癌基因。
Objective: To investigate the role and clinical significance of FHIT gene protein expression in the development and progression of laryngeal squamous cell carcinoma (LSCC). Methods: The expressions of FHIT protein in 52 cases of laryngeal carcinoma, 23 cases of adjacent tissues and 10 cases of normal tissues were detected by immunohistochemical SP method. The expression of FHIT protein was analyzed under different clinicopathological parameters. Results: The positive rate of FHIT protein in normal tissues (90.0%) and adjacent normal tissues (78.3%) were higher than that in cancerous tissues (46.2%), with significant difference (P <0.01). The positive rate of FHIT protein in cancer tissues was related to clinical stage and cervical lymph node metastasis, but not to pathological grade. Conclusions: The occurrence and development of laryngeal carcinoma are closely related to the inactivation of FHIT tumor suppressor gene.FHIT gene is likely to be an important candidate tumor suppressor gene in laryngeal cancer.