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目的:探讨乳腺癌患者术前外周血清中血管内皮生长因子-C(sVEGF-C)水平及术后肿瘤组织中VEGF-C的表达及淋巴管密度(LVD)的水平与肿瘤淋巴转移的关系。方法:ELISA法检测乳腺癌患者术前外周血清VEGF-C水平,免疫组化SP法检测乳腺癌术后肿瘤组织VEGF-C及抗淋巴管内皮透明质酸受体-1(LYVE-1)的表达,并计算对比分析肿瘤组织LVD。结果:乳腺癌患者sVEGF-C值〔(48.30±23.98)ρg/mL〕较乳腺良性病患者〔(6.26±2.37)ρg/mL〕和健康女性〔(4.72±2.09)ρg/mL〕高,组间比较P值均为0.000;sVEGF-C水平与组织VEGF-C表达正相关,VEGF-C阳性表达者sVEGF-C值为(60.75±11.12)ρg/mL,VEGF-C阴性表达者为(29.86±4.47)ρg/mL,差异有统计学意义,P=0.035;sVEGF-C水平、VEGF-C在组织中的表达分别与乳腺癌患者HER-2表达以及腋淋巴结转移相关,P<0.05。VEGF-C、LVD在乳腺癌组织中的表达较乳腺良性病高,LVD与腋淋巴结转移正相关(P=0.018),VEGF-C与LVD正相关,P=0.006。结论:乳腺癌患者术前外周血清VEGF-C和术后肿瘤组织LVD高水平在肿瘤淋巴转移中起到重要的作用。
Objective: To investigate the relationship between the levels of vascular endothelial growth factor-C (sVEGF-C) and the expression of VEGF-C and lymphatic vessel density (LVD) in the peripheral blood of patients with breast cancer before operation and their relationship with tumor lymphatic metastasis. Methods: The levels of VEGF-C in preoperative serum of breast cancer patients were detected by ELISA. The levels of VEGF-C and LYVE-1 in breast cancer were detected by immunohistochemical SP method Expression and comparative analysis of tumor tissue LVD. Results: The sVEGF-C values in patients with breast cancer 〔(48.30 ± 23.98) ρg / mL〕 were significantly higher than those in patients with benign breast disease 〔(6.26 ± 2.37) ρg / mL〕 and healthy women 〔(4.72 ± 2.09) ρg / The value of sVEGF-C was positively correlated with the expression of VEGF-C, the expression of sVEGF-C in VEGF-C was (60.75 ± 11.12) ρg / mL and the expression of VEGF-C was ± 4.47) ρg / mL, the difference was statistically significant (P = 0.035). The expression of sVEGF-C and VEGF-C were correlated with HER-2 expression and axillary lymph node metastasis in breast cancer patients, P <0.05. The expression of VEGF-C and LVD in breast cancer tissues was higher than that in benign breast diseases. LVD was positively correlated with axillary lymph node metastasis (P = 0.018). VEGF-C was positively correlated with LVD (P = 0.006). Conclusion: The preoperative serum VEGF-C levels in breast cancer patients and the high level of LVD in tumor tissues play an important role in tumor lymphatic metastasis.