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已发现吸烟妇女尿中内生性雌激素水平低于不吸烟者,但机理不清。为进一步了解香烟是否能直接影响卵巢雌激素的产生,作者研究了香烟烟雾的水溶性提取物对人类粒层细胞芳香化酶的影响。粒层细胞采自28位准备作体外授精而行诱发排卵的月经周期正常的妇女。用克罗米酚和HCG刺激卵巢滤泡发育。在给予HCG(10,000IU)34小时后,经腹腔镜吸取可达到的滤泡,用50%渗透梯度层析法使粒层细胞与红细胞分离。并将其加入塑料多孔培养扳的孔穴中,每孔细胞密度为30,000个,除特别指出外,本文各组培养基中均加入5%的小牛血清,于37℃含5%的CO_2卵箱中培养24小时,然后在实验组的培养基中加入香烟烟雾提取物再培养24小
Endogenous estrogen levels in the urine of smokers have been found to be lower than in nonsmokers, but the mechanism is unclear. To further understand whether cigarettes can directly affect the production of ovarian estrogen, the authors studied the effect of water-soluble extracts of cigarette smoke on human granulosa cell aromatase. Granulosa cells were collected from 28 women who were ready for IVF and who had normal menstrual cycles to induce ovulation. Oral follicular development is stimulated with clomiphene and HCG. After 34 hours of administration of HCG (10,000 IU), the achievable follicles were aspirated by laparoscopy and the granulosa cells and erythrocytes were separated by 50% osmotic gradient chromatography. And added to the hole of the plastic porous culture plate, the cell density per hole was 30,000. Unless otherwise specified, 5% fetal bovine serum was added into each medium group in this study, and 5% CO 2 oocyte In training for 24 hours, and then added to the experimental group of medium tobacco smoke extract and then cultured for 24 hours