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目的:观察中枢nesfatin-1对大鼠夜间摄食和胃排空的影响。方法:大鼠经腹腔注射硫酸仲丁巴比妥(100~150 mg/kg)麻醉,侧脑室、第四脑室或小脑延髓池注射nesfatin-1或CRF受体拮抗剂astressin-B或astressin2-B,观察对摄食、胃排空的影响。结果:侧脑室注射nesfatin-1后大鼠第3-6 h夜间进食量(t=3.05~3.58,P<0.01)和3 h和6 h的累积进食量(t=5.90~12.1,P<0.01)明显减少,nesfatin-1的该抑制效应可被预先侧脑室注射astressin-B或astressin2-B阻断(t=1.06~2.22,P<0.05)。第四脑室或小脑延髓池注射nesfatin-1后大鼠夜间摄食量在第1h就明显减少(t=2.59~6.26,P<0.05~0.01),持续减少至5-6h(t=1.69~7.42,P<0.05~0.01)。侧脑室注射不同剂量nesfatin-1(0.05或0.5μg)20 min后GE率明显降低,且随注射剂量增大,GE率越低(t=3.25~4.67,P<0.01)。若预先给予大鼠CRF受体拮抗剂astressin2-B(30μg)再注射nesfatin-1(0.5μg),nesfatin-1抑制大鼠胃排空效应明显减弱(t=2.45~2.85,P<0.05)。禁食24 h后再喂食2 h,大鼠下丘脑中nesfatin-1表达明显增加(t=2.87,P<0.05),禁食24 h后血浆nesfatin-1水平明显降低(t=1.51,P<0.05)。结论:Nesfatin-1抑制摄食作用可能由nesfatin-1和CRF2信号系统共同调节。
Objective: To observe the effect of central nesfatin-1 on nocturnal feeding and gastric emptying in rats. Methods: Rats were injected intraperitoneally with sophoridib (100-150 mg / kg), intracerebroventricular, fourth ventricle or cerebellar cisterna injection nesfatin-1 or CRF receptor antagonist astressin-B or astressin2-B , Observe the impact on food intake, gastric emptying. Results: After intratracheal injections of nesfatin-1, the rats’ food intake (t = 3.05-3.58, P <0.01) and the cumulative food intake at 3 h and 6 h (t = 5.90-12.1, P <0.01 ). The inhibitory effect of nesfatin-1 could be blocked by intracerebroventricular injection of astressin-B or astressin2-B (t = 1.06-2.22, P <0.05). The rats’ nocturnal food intake decreased significantly (P <0.05 ~ 0.01, t = 2.59-6.26, P <0.05 ~ 0.01) and decreased continuously to 5-6h (t = 1.69-7.42, P <0.05 ~ 0.01). After intracerebroventricular injection of nesfatin-1 (0.05 or 0.5 μg) at different doses for 20 min, the GE rate decreased significantly, and the GE rate decreased with increasing dose (t = 3.25-4.67, P <0.01). The effect of nesfatin-1 on gastric emptying in rats was significantly weakened (t = 2.45-2.85, P <0.05) when nesfatin-1 (0.5μg) was pretreated with rat CRF receptor antagonist astressin2-B (30μg) The fasting serum nesfatin-1 expression was significantly increased in the hypothalamus of rats (t = 2.87, P <0.05) after fasting for 24 h and then for 2 h, 0.05). Conclusion: The inhibitory effect of Nesfatin-1 on feeding may be regulated by nesfatin-1 and CRF2 signaling systems.