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目的:观察脂多糖(LPS)干预下,不同浓度羧甲基壳聚糖(carboxymethyl chitosan,CMC)对人牙周膜成纤维细胞(HPDLFs)表达前列腺素E2(PGE2)的影响。方法:取冻存的人牙周膜成纤维细胞进行复苏培养,以浓度为10mg/L的LPS进行诱导,用不同浓度的CMC进行干预,采用免疫组织化学方法检测PGE2在HPDLFs中的表达变化。结果:不同浓度CMC能够下调同一浓度LPS诱导的HPDLFs表达的PGE2,随CMC浓度的增加PGE2的表达量呈逐渐减弱趋势(P<0.05)。结论:CMC可能对LPS诱导的HPDLFs炎症损伤过程中表达PGE2有重要的抑制作用。
OBJECTIVE: To observe the effects of different concentrations of carboxymethyl chitosan (CMC) on the expression of prostaglandin E2 (PGE2) in human periodontal ligament fibroblasts (HPDLFs) treated with lipopolysaccharide (LPS). Methods: Frozen human periodontal ligament fibroblasts were resuscitated and cultured in LPS at a concentration of 10 mg / L. The cells were treated with different concentrations of CMC. The expression of PGE2 in HPDLFs was detected by immunohistochemistry. Results: Different concentrations of CMC could down-regulate PGE2 expression induced by LPS-induced HPDLFs. PGE2 expression gradually decreased with the increase of CMC concentration (P <0.05). Conclusion: CMC may have an important inhibitory effect on the expression of PGE2 during LPS-induced HPDLFs inflammatory injury.