目的:探讨鼠尾悬挂试验(TST)在大鼠急性坏死性胰腺炎中的实验意义。方法:SD大鼠被分为三组:急性坏死性胰腺炎组(A组,n=8)、急性坏死性胰腺炎鱼油干预组(F组,n=8)和对照组(C组,n=8)。观察各组大鼠胰腺组织病理变化及TST静止时间,同时测定各组5d后血清SOD、GSH-Px活力和淀粉酶浓度。结果:A组、F组TST静止时间各时间段均显著高于C组(P<0.01);自第2天起F组的数值均小于A组,其中第4、第5天的数值差异有显著性(分别为P<0.05、P<0.01)。病理学检查提示F组的炎性细胞浸润显著低于A组(P<0.01)。A组SOD、GSH-Px显著低于C组(P<0.01),而F组SOD、GSH-Px显著高于A组(P<0.01)。TST静止时间和病理学改变、SOD、GSH-Px活力具有相关性(P<0.01)。结论:TST静止时间在一定程度上反映了急性坏死性胰腺炎的严重程度;鱼油在改善急性坏死性胰腺炎大鼠的氧化应激指标的同时,对大鼠的TST静止时间产生影响。 Objective: To investigate the experimental significance of rat tail suspension test (TST) in acute necrotizing pancreatitis in rats. Methods: The SD rats were divided into three groups: acute necrotizing pancreatitis group (group A, n = 8), acute necrotizing pancreatitis fish oil intervention group (group F, n = 8) and control group = 8). The pathological changes of pancreatic tissue and the resting time of TST in each group were observed. At the same time, the activities of SOD, GSH-Px and the concentration of amylase in serum of the rats in each group were measured 5 days later. Results: The resting time of TST in group A and group F were significantly higher than those in group C (P <0.01), and the values ​​of group F were less than those in group A Significance (P <0.05, P <0.01, respectively). Pathological examination showed that inflammatory cell infiltration in group F was significantly lower than that in group A (P <0.01). The SOD and GSH-Px in group A were significantly lower than those in group C (P <0.01), but the levels of SOD and GSH-Px in group F were significantly higher than those in group A (P <0.01). TST still time and pathological changes, SOD, GSH-Px activity was correlated (P <0.01). Conclusion: The resting time of TST to a certain extent reflects the severity of acute necrotizing pancreatitis; fish oil in improving acute necrotizing pancreatitis in rats with oxidative stress indicators at the same time, the impact on rat TST resting time.