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目的：研究神经生长因子（ＮＧＦ）急性毒性及长期毒性。方法：小鼠急毒按最大耐受量测定法进行大鼠长期毒性试验时分低（５０００ＢＵ／ｋｇ）、中（７０７０ＢＵ／ｋｇ）、高（１００００ＢＵ／ｋｇ）３个试验组和１个生理盐水对照组；ｉｍ，共９０天。部分动物给药结束后继续观察１４天。结果：小鼠一次ｉｐ、ｉｍ、ｉｖＮＧＦ的最大耐受量均＞２５０００ＢＵ／ｋｇ，大鼠连续ｉｍＮＧＦ９０天，给药期间、给药结束及停药１４天时血液学、血液生化、病检均无异常所见，不影响大鼠行为和生长发育。结论：ＮＧＦ对小鼠一次性ｉｖ、ｉｐ、ｉｍ的最大耐受量大于２５０００ＢＵ／ｋｇ，９０天肌肉内注射给药，对大鼠无明显毒性。
Objective: To study the acute toxicity and long-term toxicity of nerve growth factor (NGF). Methods: The acute toxicity of mice was determined according to the method of maximum tolerated dose. Long-term toxicity test was performed in rats at low dose (5000BU / kg), medium dose (7070BU / kg) and high dose (10000BU / Control group; im, a total of 90 days. Some animals continue to observe after 14 days of administration. Results: The maximum tolerated dose of ip, im and ivNGF in mice was> 25000BU / kg and the imNGF was continuously administered to rats for 90 days. No abnormalities in hematology, blood biochemistry and pathology were observed during the administration, 14 days after drug administration and 14 days after drug withdrawal See, does not affect rat behavior and growth and development. CONCLUSION: The maximum tolerated dose of NGF for mice once iv, ip, im is more than 25000BU / kg, administered intramuscularly for 90 days without obvious toxicity to rats.