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目的：建立人子宫颈癌裸鼠移植瘤株，为开展宫颈癌的实验研究奠定基础。方法：将子宫颈高分化鳞癌手术标本移植于裸鼠皮下，生长后，于鼠间连续传代，并检测移植瘤的相关生物学特征；进行光镜、电镜观察和染色体分析；用免疫组化技术检测ＥＲ、ＰＲ、Ｋｅｒａｔｉｎ、ＣＥＡ、ＰＣＮＡ，Ｐ５３、ｎｍ２３、ＨＥＲ－２；用多聚酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）检测ｈＰＶ１６、１８、ＨＳＶ－Ⅱ、ＣＭＶ；用流式细胞术检测ＤＮＡ含量；进行铂类抗癌药物敏感试验以及癌细胞的体外培养等。结果：历时１７个月，传了２６代，建成人子宫颈高分化鳞癌裸鼠移植瘤株，命名为ｈＣＣ－９４Ｖ。瘤株生长稳定，潜伏期１６～２０ｄ，移植成功率９２．９％，浸润转移率１１．１％。光镜、电镜下形态学与患者肿瘤结构特征一致。染色体多为异倍体，总数为９～１２０条。免疫组化检测肿瘤标记物（ＴＭ）呈高表达，癌基因蛋白呈低表达。ＰＣＲ检测ｈＰＶ１６（＋），对铂类抗癌药物敏感。用移植瘤建成体外培养细胞系ｈＣＣ－９４Ｖ及其亚株。结论：ｈＣＣ－９４为进一步进行子宫颈癌的实验研究提供了理想的动物模型和瘤源
Objective: To establish a transplanted tumor strain of human cervical cancer in nude mice and lay a foundation for the experimental study of cervical cancer. METHODS: Surgical specimens of well-differentiated squamous carcinoma of the cervix were transplanted subcutaneously in nude mice. After growth, serial passages were performed between mice. The relevant biological characteristics of the transplanted tumors were examined. Light microscopy, electron microscopy, and chromosome analysis were performed. Immunohistochemistry was performed. Detection of ER, PR, Keratin, CEA, PCNA, P53, nm23, and HER-2; detection of hPV16, 18, HSV-II, and CMV by polymerase chain reaction (PCR); detection of DNA content by flow cytometry; Platinum anticancer drug sensitivity test and in vitro culture of cancer cells. RESULTS: A total of 26 passages were passed for 17 months and a transplanted tumor of human cervix well-differentiated squamous cell carcinoma was established and named as hCC-94V. The tumor growth was stable, the incubation period was 16 to 20 days, the success rate of transplantation was 92.9%, and the infiltration transfer rate was 11.1%. Light microscopy, electron microscopy morphology consistent with the characteristics of the patient’s tumor structure. The chromosomes are mostly aneuploid and the total number is 9 to 120. Immunohistochemistry showed high expression of tumor markers (TM) and low expression of oncogene proteins. Detection of hPV16(+) by PCR was sensitive to platinum anticancer drugs. The in vitro cultured cell line hCC-94V and its sub-strains were established with xenografts. Conclusion: hCC-94 provides an ideal animal model and tumor source for further experimental studies of cervical cancer