淋巴毒素是淋巴细胞产生的一组淋巴激活素,是一些等电点为4.5—5.5的酸性糖蛋白。分子量12,000—1,200,000.淋巴毒素可以用有丝分裂原或抗原刺激自细胞产生。作者的方法是把来自人或实验动物淋巴结、周围血、脾的淋巴样细胞与有丝分裂原或抗原在无血清培养基中共培养24小时,然后收集无细胞培养液。用Amicon YM10膜过滤、浓缩,通过等电聚 Lymphtoxins are a group of lymphokines produced by lymphocytes and are acid glycoproteins with isoelectric points of 4.5-5.5. Molecular weight 12,000-1,200,000. Lymphotoxin can be generated from cells by stimulation with mitogen or antigen. The authors’ method is to co-culture lymphoid cells from lymph nodes, peripheral blood, and spleen of human or experimental animals with mitogens or antigens for 24 hours in serum-free medium and then collect the cell-free culture medium. Filtered with Amicon YM10 membrane, concentrated, isoelectric-charged