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目的探讨全氟辛酸(perfluorooctanoic acid,PFOA)对新生大鼠星形胶质细胞(astrocytes,As)内游离钙离子浓度([Ca2+]i)的影响。方法星形胶质细胞取材于新生24 h内清洁级Wistar大鼠。传代细胞达到80%融合时进行染毒(PFOA终浓度分别为0、50、100、200、500μmol/L)。以Fura-2/AM荧光探针法测定PFOA染毒1、2、3 h后星形胶质细胞内的([Ca2+]i)。结果星形胶质细胞内的[Ca2+]i随着PFOA剂量的增加和染毒时间的延长而增加,染毒1 h时500μmol/L剂量组星形胶质细胞内的[Ca2+]i为353.57 nmol/L,染毒2 h时达到528.37 nmol/L,均高于对照组(161.86、155.03 nmol/L),差异均有统计学意义(P<0.01)。当染毒3 h时,50、100、200、500μmol/L剂量组星形胶质细胞内的[Ca2+]i分别为320.93、396.43、601.26、476.20 nmol/L,均显著高于对照组(164.62 nmol/L)(P<0.01),同时也显著高于相同剂量染毒1 h的[Ca2+]i(P<0.01)。结论 PFOA可通过增加新生大鼠星形胶质细胞内[Ca2+]i而影响中枢神经系统钙平衡。
Objective To investigate the effect of perfluorooctanoic acid (PFOA) on free calcium concentration ([Ca2 +] i) in astrocytes (As) of neonatal rats. Methods Astrocytes were taken from clean-grade Wistar rats within 24 hours after birth. The passaged cells were exposed to 80% confluency (final concentrations of PFOA were 0, 50, 100, 200 and 500 μmol / L, respectively). Fura-2 / AM fluorescent probe method was used to determine the intracellular ([Ca2 +] i) of astrocytes after exposure to PFOA for 1, 2 h. Results The [Ca2 +] i in astrocytes increased with the dose of PFOA and the prolongation of exposure time. [Ca2 +] i in astrocytes at 500 μmol / L for 1 h was 353.57 nmol / L, reached 528.37 nmol / L at 2 h after exposure, both of which were higher than that of the control group (161.86 and 155.03 nmol / L, respectively), with statistical significance (P <0.01). When exposed to 3 h, the [Ca2 +] i in the astrocytes were 320.93, 396.43, 601.26 and 476.20 nmol / L, respectively, which were significantly higher than those of the control group (164.62 nmol / L) (P <0.01), and also significantly higher than [Ca2 +] i at the same dose for 1 h (P <0.01). Conclusion PFOA can affect the central nervous system calcium balance by increasing [Ca2 +] i in astrocytes of neonatal rats.