[目的]建立测定复方杠板归胶囊中芍药苷含量的高效液相色谱法。[方法]采用Sinochrom ODS-AP柱(5μm,4.6×250 mm),流动相为甲醇-0.3%磷酸溶液(35∶65),柱温30℃,流速0.8 ml/min,检测波长230 nm。[结果]芍药苷含量在0.0405 4-0.243 2μg/μl(r=0.999 99)与峰面积呈良好线性关系,平均加样回收率为98.89%(RSD为2.17%)。[结论]该方法快捷,方便,重复性好,可作为该复方的质量控制指标。 [Objective] To establish a HPLC method for the determination of paeoniflorin in Fufangbaipian capsule. [Method] The column of Sinochrom ODS-AP (5μm, 4.6 × 250 mm) was used. The mobile phase was methanol-0.3% phosphoric acid solution (35:65). The column temperature was 30 ℃ and the flow rate was 0.8 ml / min. [Results] Paeoniflorin had a good linear relationship with the peak area at 0.0405 4-0.243 2 μg / μl (r = 0.999 99), with an average recovery of 98.89% (RSD 2.17%). [Conclusion] The method is fast, convenient and reproducible, which can be used as quality control index of this compound.