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目的分析SLE患者外周血单一核细胞TH2型细胞因子mRNA的表达状况.方法分离SLE外周血单一核细胞1×107/mL的浓度,进行总RNA提取,然后用1.5%的琼脂糖电泳,应用凝胶成像系统对电泳条带进行扫描.结果SLE患者IL-4mRNA,IL-6mRNA和IL-10mRNA水平相比对照组显著升高.结论TH2型细胞因子受体基因的表达在SLE发病机制中有明显的异常,其与相应蛋白表达的关系还有待进一步探讨.
Objective To analyze the expression of TH2 cytokine mRNA in peripheral blood mononuclear cells of patients with SLE.Methods The concentration of 1 × 107 / mL SLE peripheral blood mononuclear cells was isolated and the total RNA was extracted, and then 1.5% agarose gel electrophoresis Gel system.Results The levels of IL-4 mRNA, IL-6 mRNA and IL-10 mRNA in SLE patients were significantly increased compared with the control group.Conclusion The expression of TH2 cytokine receptor gene is obviously in the pathogenesis of SLE Abnormal, its relationship with the expression of the corresponding protein remains to be further explored.