论文部分内容阅读
目的 :探讨血管紧张素Ⅱ受体I(AT1 )的A116 6C基因多态性与糖尿病肾病的相关性 ;AT1 的A116 6C基因多态对Ⅱ型糖尿病肾病发病因素的影响。方法 :用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析法探查AT1 的A116 6C基因多态性在健康组、单纯Ⅱ型糖尿病组 (以下简称DM组 )、糠尿病肾病组 (以下简称DN组 )的基因频率分布 ,并分析其对以上疾病发病因素的影响。结果 :各组之间并未发现明显不同的等位基因分布 ;各组内AT1基因型与各因素间的单因素偏相关分析表明 :DM组中AC基因型与空腹胰岛素水平 (FINS)和血清甘油三酯 (TG)呈正相关 (r1 =0 .42 9 ,r2 =0 .2 5 5 ) ;DN组中AC基因型与脂蛋白 (a)呈正相关 (r=0 .318 )。结论 :提示AT1 基因A116 6C多态与糖尿病肾病无关 ,但携带C等位基因的患者易于发生脂代谢紊乱
Objective: To investigate the relationship between A116 6C gene polymorphism of angiotensin Ⅱ receptor I (AT1) and diabetic nephropathy and the effect of A116 6C gene polymorphism of AT1 on the pathogenesis of type Ⅱ diabetic nephropathy. Methods: Polymorphisms of A116 6C gene in AT1 were detected by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) analysis in healthy group, simple type Ⅱ diabetes mellitus group (hereinafter referred to as DM group), nephropathy group (Hereinafter referred to as DN group) gene frequency distribution, and analyze its impact on the incidence of these diseases. Results: No significant allele distribution was found among the groups. One-way partial correlation analysis between AT1 genotype and each factor in each group showed that AC genotype and fasting insulin (FINS) and serum Triglyceride (TG) was positively correlated (r1 = 0.42 9, r2 = 0.255). There was a positive correlation between AC genotypes and lipoprotein (a) in DN group (r = 0.338). Conclusion: It is suggested that A116 6C polymorphism of AT1 gene is not associated with diabetic nephropathy, but patients with C allele are prone to lipid metabolism disorders