论文部分内容阅读
将栽培种Solanum meolngena L.中的竹丝茄嫁接于野生牛茄子(Solanum surattense Burm.f)上,于幼苗期、结果后期取顶部茎叶和根,用垂直板聚丙烯酸胺凝胶电泳分析其过氧化物酶(POD)同工酶.幼苗期,嫁接苗与接穗苗植株茎叶的POD均有4条酶带,但强弱和相对电泳迁移率(R_1)略有差异;在根部则嫁接苗与砧木苗的酶带相同,而与接穗苗有差异.结果后期,嫁接植株茎叶的POD同工酶发生变化,有5条酶带,比接穗苗增加1条,酶带的强弱和R_1也发生了变化;嫁接苗根的POD酶带谱与砧木苗相同,仍为7条酶带,但酶带的强弱及R_1有差异.由此可见,嫁接植株POD同工酶的变化是接穗和砧木相互影响的结果,这种酶谱的变化可能是茄子嫁接植株抗病性增强在生理上的反应.
The Solanum meolngena L. cultivated species Solanum melongena L. was grafted onto Solanum surattense Burm.f. At the seedling stage, the top stems, leaves and roots were harvested at the end of the experiment and were analyzed by vertical plate polyacrylamide gel electrophoresis Peroxidase (POD) isoenzyme. There were 4 bands of POD in stems and leaves of grafted seedlings and scion seedlings at seedling stage, but there was a slight difference in the strength and relative electrophoretic mobility (R_1) The results showed that the POD isozymes of the stems and leaves of the grafted plants changed in the late stage, with five bands, one more than the scion, the strength of the bands and R_1 also changed; POD enzyme bands of grafted seedling roots were the same as that of rootstock seedlings, but there were still seven bands, but the bands were different and the R_1 was different.Therefore, the changes of POD isozymes in grafted plants were As a result of the interaction between scion and rootstock, the change of this zymogram may be the physiological response of the grafted eggplant to enhance disease resistance.