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目的探讨病毒特异性CTL对HIVHCV共感染患者病情进展的影响机制。方法观察对象为HIVHCV共感染患者、单纯HIV感染者、单纯HCV感染者。采用四聚体技术,运用流式细胞仪检测病毒特异性CTL。前瞻性比较HIV、HCV特异性CTL在三组中的异同,并对HIVHCV共感染患者的HIV、HCV特异性CTL进行相关性分析。结果HIVHCV共感染组与单纯HIV感染组比较HIV特异性CTL,差异无显著性(P=0.586)。HIVHCV共感染组中HCV特异性CTL的百分数及绝对计数(0.37±0.29,3.52±3.79)均高于单纯HCV感染组(0.15±0.05,0.86±0.33),差异有统计学意义(P=0.001,P=0.002)。HIVHCV共感染组中的HIV特异性CTL与HCV特异性CTL存在正性线性相关(P<0.001),方程成立。并且各系数均有统计学意义,方程似然比(r2)0.761。结论HCV特异性CTL可能是HIVHCV共感染组中肝脏功能损伤加重的原因之一;HIV与HCV在同一患者存在相互影响。
Objective To investigate the effect of virus-specific CTL on the progression of HIV-co-infected patients. Methods The subjects were HIV-HCV co-infected patients, HIV-infected individuals and HCV-infected individuals. Tetramer technology was used to detect virus-specific CTL using flow cytometry. Prospective comparison of HIV and HCV-specific CTL in three groups of similarities and differences, and HIV-HCV co-infected patients with HIV, HCV-specific CTL correlation analysis. Results There was no significant difference in HIV-specific CTL between HIV-HCV co-infected group and HIV-infected group (P = 0.586). The percentage of HCV-specific CTLs and absolute counts (0.37 ± 0.29, 3.52 ± 3.79) in HIV-HCV co-infected patients were significantly higher than those in HCV-infected patients (0.15 ± 0.05,0.86 ± 0.33) (P = 0.001, P = 0.002). There was a positive linear correlation (P <0.001) between HIV-specific CTLs and HCV-specific CTLs in the HIV-HCV co-infected group. The equation was established. And the coefficients were statistically significant, the equation likelihood ratio (r2) 0.761. Conclusion HCV-specific CTL may be one of the causes of liver dysfunction in HIV-HCV co-infected group. There is interaction between HIV and HCV in the same patient.