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用苹果轮纹病菌菌株Ls1的菌丝接种苹果抗病品种‘红玉’和感病品种‘金冠’及其杂种后代高抗单株02-24-036和高感单株02-38-071的果实,采用扫描和透射电镜技术观察其表面及皮下组织亚显微结构的变化情况。结果发现,病菌侵染后,红玉、金冠和02-24-036果面均产生特殊的分泌物,其中红玉和02-24-036产生的分泌物抑制菌丝繁殖,金冠果面产生的分泌物不能抑制菌丝繁殖。说明病菌侵染后果面产生的分泌物与抑菌和抗侵入有关。菌丝由果面皮孔或伤口侵入组织后,抗病的红玉和02-24-036皮下细胞内嗜锇颗粒簇积累,而感病的金冠和02-38-071未见嗜锇颗粒簇的积累。说明红玉和02-24-036果实对苹果轮纹病的抗性属于诱导抗性,既表现为抗侵入,也表现为抗扩展。
The resistant varieties “Hongyuanyu” and susceptible cultivar ’Golden Delicious’ and their offspring of their resistant offspring were inoculated with the mycelia of apple rotten pathogen Ls1 to high resistance lines 02-24-036 and susceptible lines 02-38-071 The changes of sub-microstructure on the surface and subcutaneous tissue were observed by scanning and transmission electron microscopy. The results showed that after the pathogen infection, ruby, golden crown and 02-24-036 fruit surface produce special secretions, of which ruby and 02-24-036 secretions inhibit mycelial growth, golden crown fruit produced Secretions can not inhibit mycelial growth. Explain the germ infection after infection and the resulting surface antimicrobial and anti-invasive. After mycelium invaded tissues from the bark or wounds of the fruit, disease-resistant ruby and accumulation of osmiophilic granules in the subcutaneous cells of 02-24-036 accumulated in the cells, while the susceptible golden crowns and 02-38-071 showed no osmiophilic granules accumulation. The results indicated that the resistance of Rhododendron and 02-24-036 to apple grain rot disease was induced resistance, showing anti-invasion and anti-expansion.