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目的探讨别嘌呤醇对未成熟鼠脑白质损伤的保护作用。方法将新生1日龄SD大鼠84只随机分为假手术组(Sham)(n=28)、双侧颈总动脉结扎(BCAO)组(n=28)及别嘌呤醇干预组(ALLO)(n=28),BCAO制作脑白质损伤模型。观察BCAO后7d(P7,每组各6只)和14d(P14,每组8只)脑组织病理改变及MBP免疫组化变化;RT-PCR荧光定量法检测P7(每组6只)和P14(每组8只)脑组织MBP-mRNA的表达。结果BCAO组白质改变明显,ALLO组较之明显减轻;P14平均脑室大小指数,ALLO组较(2.44±0.71)%BCAO组(3.27±0.73)%明显减小(P<0.05)。P14MBP阳性染色光密度值,BCAO组(6.60±0.68)较Sham组(9.40±0.53)明显降低(P<0.01),而ALLO组(7.10±0.18)较BCAO组明显升高(P<0.05);P7、P14MBP-mRNA拷贝数(log10),BCAO组和ALLO组均明显低于Sham组(P<0.01),而ALLO组则较BCAO组明显升高(P<0.05)。结论利用1日龄大鼠行BCAO可较好地制作早产儿脑白质损伤模型。别嘌呤醇对缺血引起的未成熟大鼠脑白质损伤具有保护作用。
Objective To investigate the protective effect of allopurinol on immature rat brain white matter injury. Methods Eighty-four newborn SD rats aged 1 day were randomly divided into Sham group (n = 28), BCAO group (n = 28) and allopurinol group (ALLO) (n = 28), BCAO made white matter damage model. Pathological changes and MBP immunohistochemistry were observed at 7 days after BCAO (P7, 6 in each group) and 14 days (P14, 8 in each group). P7 (6 in each group) and P14 (8 in each group) brain tissue MBP-mRNA expression. Results The white matter of BCAO group changed obviously, and ALLO group was obviously relieved. The mean ventricular size index of P14 group was significantly lower than that of (2.44 ± 0.71)% BCAO group (3.27 ± 0.73)% in ALLO group (P <0.05). The P14MBP positive staining OD value in BCAO group (6.60 ± 0.68) was significantly lower than that in Sham group (9.40 ± 0.53) (P <0.01), but was significantly higher in ALLO group (7.10 ± 0.18) than that in BCAO group (P <0.05) P7, P14MBP-mRNA copy number (log10) in BCAO group and ALLO group were significantly lower than those in Sham group (P <0.01), while those in ALLO group were significantly higher than those in BCAO group (P <0.05). CONCLUSION: BCAO, a one-day old rat, can be used to make a model of white matter damage in premature infants. Allopurinol has a protective effect on white matter damage induced by ischemia in immature rats.