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小分子热激蛋白与植物耐寒性提高有相关性,但是没有直接的实验证据能证明小分子热激蛋白的存在增加植物抗寒性。我们克隆了番茄叶绿体(定位)小分子热激蛋白cDNA,并将35SCaMV启动子驱动的番茄叶绿体小分子热激蛋白cDNA植物表达构架导入番茄,测定转基因番茄和未转基因番茄的抗寒性水平。低温处理后,转基因番茄的冷害症状轻于未转基因的番茄;转基因番茄细胞电解质外渗较少、花青素和MDA累积量较低;净光合速率和叶绿体含量高于对照。这些实验结果说明叶绿体小分子热激蛋白的过量表达提高了植物抗寒性。
However, there is no direct experimental evidence that the existence of small-molecule heat-shock proteins can increase cold tolerance of plants. We cloned the small-molecule heat-shock protein cDNA of tomato chloroplast (targeting), introduced the tomato chloroplast small-molecule heat-shock protein cDNA expression vector driven by 35SCaMV promoter into tomato and determined the cold resistance of transgenic tomato and non-transgenic tomato. After chilling treatment, the symptoms of chilling injury in transgenic tomato were lighter than those in non-transgenic tomato. The electrolyte of transgenic tomato had less extravasation, lower accumulation of anthocyanin and MDA, higher net photosynthetic rate and chloroplast content than the control. These experimental results indicate that overexpression of chloroplast small-molecule heat shock protein enhances plant cold resistance.