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目的新合成活性酪氨酸磷酸酶探针,研究酪氨酸磷酸酶的差异表达,探寻利用该探针示踪检测磷酸酶,进行疾病快速诊断的可能。方法更换反应起始物,用对苯二甲醛为起始原料,优化合成路线,链接生物素用作探针标签,通过质谱鉴定,得到文献报道的活性磷酸酶探针。用胶质瘤细胞株U251、胃癌组织T366、碱性磷酸酶ALP和酪氨酸磷酸酶Yo PH验证探针的特异性和广谱性后,用胃癌细胞株MKN28、SGC7901、BGC823、HGC27等摸索试验条件,对照生物素利用乳腺癌细胞株MDA-BA-231、肝癌细胞株huh7、胃癌细胞株BGC823等细胞和胃癌、胶质瘤组织样品,探索疾病检测的新方法。结果缩减实验步骤,成功合成出活性酪氨酸磷酸酶探针。经验证,该探针具有较好的特异性,可以进行相关应用。结论运用本研究新合成的路线,快速合成出了可供临床研究应用的活性酪氨酸磷酸酶探针。
Objective To synthesize active tyrosine phosphatase probe, to study the differential expression of tyrosine phosphatase and explore the possibility of using this probe to detect phosphatase for rapid diagnosis of disease. Methods The reaction starting material was replaced with terephthalaldehyde as starting material to optimize the synthetic route. The biotin was used as a probe tag to identify the active phosphatase probe reported in the literature. The specificity and broad spectrum of the probes were verified by glioma cell line U251, gastric cancer T366, alkaline phosphatase ALP and tyrosine phosphatase Yo PH, and then probed with gastric cancer cell lines MKN28, SGC7901, BGC823, HGC27 and so on Test conditions, the control biotin using breast cancer cell lines MDA-BA-231, liver cancer cell line huh7, gastric cancer cell line BGC823 and other cells and gastric cancer, glioma tissue samples to explore new methods of disease detection. As a result, the active tyrosine phosphatase probe was successfully synthesized by reducing the experimental steps. Proved that the probe has good specificity, can be related applications. Conclusions The active tyrosine phosphatase probe for clinical research and application has been rapidly synthesized using the newly synthesized route in this study.