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目的:探讨无细胞短小棒状杆菌纳米级制剂(NCPP)对小鼠巨噬细胞的影响。方法:采用纳米技术制备NCPP。将NCPP与短小棒状杆菌分别注射小鼠腹腔,10 d后,处死小鼠,收集腹腔液体,分离培养巨噬细胞。采用流式细胞术和ELISA法,检测巨噬细胞分泌的肿瘤坏死因子(TNF-α)和干扰素(IFN-γ)。结果:流式结果显示,NCPP组小鼠腹腔巨噬细胞分泌的肿瘤坏死因子(TNF-α)明显低于短小棒状杆菌组(P<0.05)。ELISA结果显示,NCPP组小鼠腹腔巨噬细胞分泌的肿瘤坏死因子(TNF-α)明显低于短小棒状杆菌组(P<0.01),分泌的干扰素(IFN-γ)水平明显高于短小棒状杆菌组(P<0.01)。结论:NCPP对巨噬细胞具有免疫调节作用。NCPP可以刺激巨噬细胞产生TNF-α和IFN-γ。TNF-α水平比较低,可能是NCPP副作用较少的原因之一。
Objective: To investigate the effect of NCPP on mouse macrophages. Methods: NCPP was prepared by nanotechnology. The mice were injected with NCPP and Corynebacterium parvum respectively. After 10 days, the mice were sacrificed and the peritoneal fluid was collected. Macrophages were isolated and cultured. Tumor necrosis factor (TNF-α) and interferon (IFN-γ) secreted by macrophages were detected by flow cytometry and ELISA. Results: The results of flow cytometry showed that TNF-α secreted by peritoneal macrophages in NCPP group was significantly lower than that in Corynebacterium parvum group (P <0.05). The results of ELISA showed that TNF-α secreted by peritoneal macrophages in NCPP group was significantly lower than that in Corynebacterium parvum group (P <0.01), and the level of IFN-γ secreted by NCPP group was significantly higher than that of short rod Bacillus group (P <0.01). Conclusion: NCPP has immunomodulatory effect on macrophages. NCPP can stimulate macrophages to produce TNF-α and IFN-γ. Lower levels of TNF-α may be one of the reasons for the less side effects of NCPP.