论文部分内容阅读
目的通过检测角蛋白14(cytokeratin,CK)及胃泌素(Gastrin,GAS)基因在食管癌癌组织和癌旁正常组织中的表达和基因启动子甲基化分子改变,了解其在食管癌癌变过程中的变化特征和规律,阐明其在食管癌变机制中的作用。方法应用甲基化特异性PCR(methylation-specific polymerase chain reaction,MSP)对32例食管癌患者CK14及Gas基因启动子区甲基化状态进行检测;通过免疫组织化学染色SABC法对食管癌CK14及GAS蛋白表达情况进行检测。结果32例食管癌组织中有7例检测出CK14基因启动子区异常甲基化,甲基化阳性率为22%(7/32)。32例食管癌组织中有5例检测出Gas基因启动子区异常甲基化,甲基化阳性率为16%(5/32)。32例食管癌中细胞角蛋白表达强阳性为19例(59.3%),癌旁食管上皮组织4例(12.5%)CK14表达。32例食管癌中胃泌素表达强阳性为Gas 23例(71.8%),癌旁食管上皮组织3例(9.3%)Gas表达。结论 CK14及Gas蛋白的异常表达可能是食管癌变机制之一;CK14及Gas基因启动子低甲基化可能导致蛋白的高表达可能是食管癌癌变的发病机制之一。
Objective To detect the expression of cytokeratin (CK) and gastrin (GAS) gene in esophageal cancer tissues and adjacent normal tissues and the methylation of gene promoter in esophageal carcinoma The process of changes in the characteristics and rules to clarify its role in the mechanism of esophageal cancer. Methods Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation status of CK14 and Gas gene in 32 patients with esophageal cancer. The immunohistochemical staining of SABC was used to detect the expression of CK14 and GAS protein expression was detected. Results Among the 32 cases of esophageal cancer, 7 cases had abnormal methylation of promoter region of CK14, and the positive rate of methylation was 22% (7/32). In 32 cases of esophageal cancer tissues, 5 cases detected abnormal methylation of Gas gene promoter region, the methylation positive rate was 16% (5/32). In 32 cases of esophageal cancer, the expression of cytokeratin was strongly positive in 19 cases (59.3%) and 4 cases (12.5%) of the para-carcinoma esophageal epithelium. In 32 cases of esophageal cancer, the strong positive expression of gastrin was Gas (23 cases, 71.8%), and paracancerous esophageal epithelium (3 cases, 9.3%), Gas expression. Conclusions The abnormal expression of CK14 and Gas may be one of the mechanisms of esophageal carcinogenesis. The hypomethylation of CK14 and Gas gene may lead to the high expression of protein may be one of the pathogenesis of carcinogenesis of esophageal cancer.