简要介绍了作者近 2 0年来对种子吸胀冷害和渗透调控深入而系统的研究所取得的主要成果 ,包括种子吸胀冷害发生的规律及机理 ,生物膜“双修补”理论和完善的“渗控”系统的确立及其应用效果。以多层次系列试验的直接证据确证种子吸胀冷害的原初作用位点———靶是在生物膜上。着重阐述作为种子吸胀冷害的信号———适应性保卫反应与其三个类型的发现 ,以及种子低温吸胀“双修补”理论的内容及其重要意义和实用价值 This paper briefly introduces the main achievements of the author in the recent 20 years on the systematic and in-depth study on the regulation of osmotic expansion and infiltration of seed, including the law and mechanism of seed absorption and chilling injury, the theory of “double repair” of biofilm, Control “system and its application effect. Direct evidence from multilevel series experiments confirms that the primary site of chilling injury in seed - the target is on the biofilm. It focuses on the signal of chilling injury of seed - adaptive defense response and its discovery of three types, and the content of ”double repair" theory of seed low temperature swelling and its significance and practical value