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应用免疫组织化学方法检测230例胃粘膜病变中增殖细胞核抗原（PCNA）的表达情况，计算细胞增殖指数（PI）、探讨胄粘膜肠化生的增殖状态分型以及肠化生细胞增殖分型与肿瘤相关基因蛋白产物的关系。结果显示正常或慢性浅表性胃炎、伴肠化生慢性萎缩性胃炎、伴异型增生萎缩性胃炎以及胃腺癌的PI逐渐增高，差异非常明显（P＜0．0）。根据胃粘膜肠化生细胞的PI分布，进行畅化生细胞的增殖状态分型：高增殖状态型为PI≥40，中增殖状态型为20≤P＜40，低增殖状态型为PI＜20。此分型与各类胃粘膜病变显示较好的相关性，低增殖状态型为正常或基本正常胃粘膜，中增殖状态型为胃粘膜良性病变，高增殖状态型为胃粘膜癌前病变。高增殖状态型肠化生中肿瘤相关基因蛋白产物rasp21、EGFR、h-met的表达以及叵型肠化生的检出率显著高于中、低增殖状态型肠化生（P＜0．05）。提示高增殖状态型肠化生细胞基因表达的异常率增高，且细胞分化不良，更具胃癌前病变的特征。
Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen (PCNA) in 230 cases of gastric mucosal lesions. The cell proliferation index (PI) was calculated, and the proliferative status classification of intestinal metaplasia and the proliferation of intestinal metaplasia were explored. The relationship between tumor-associated gene protein products. The results showed that the PI of normal or chronic superficial gastritis, chronic atrophic gastritis with intestinal metaplasia, atypical hyperplasia atrophic gastritis, and gastric adenocarcinoma was gradually increased, and the difference was very significant (P<0.0). According to the distribution of PI in the intestinal metaplasia of gastric mucosa, the proliferative state of the mobilized progenitor cells is classified: PI≥40 in the hyperproliferation state, 20≤P<40 in the middle proliferative state, and PI<20 in the low proliferative state. . This classification showed good correlation with various types of gastric mucosal lesions. The low proliferative state was normal or essentially normal gastric mucosa, the proliferative state was benign gastric lesions, and the hyperproliferative state was gastric precancerous lesions. The expression of tumor-associated gene protein products rasp21, EGFR, h-met and intestinal metaplasia in hyperproliferative state of intestine metaplasia were significantly higher than those in metaplasia (P<0.05). ). It is suggested that the abnormal rate of gene expression in the hyperproliferative state intestinal metaplasia cells is increased, and the cell differentiation is poor, which is more characteristic of precancerous lesions.