近年来,棉酚在治疗子宫内膜异位和某些功能性出血方面,取得了较好疗效,是一个有希望的药物。但因其分子中有六个酚羟基和二个醛基,很不稳定,此外还可能含有从棉籽提取过程中混入的杂质,故建立有效的分析手段实属必要。关于棉酚中杂质的分析已有不少报道,但都各有弊端。近来,高效液相色谱法,已日益被广泛应用。但因棉酚中所含酚羟基较多,寻找理想的色谱条件较困难,Abou-Donia等报道,在十八烷基键合相μBondapak C_(18)色谱柱上,以含0.1％磷酸的80％甲醇作流动相分离鉴定棉酚之纯度,Fong等 In recent years, gossypol has achieved good results in the treatment of endometriosis and certain functional bleeding, and is a promising drug. However, since there are six phenolic hydroxyl groups and two aldehyde groups in the molecule, they are very unstable and may contain impurities that have been incorporated from the cottonseed extraction process. Therefore, it is necessary to establish an effective analysis method. There have been many reports on the analysis of the impurities in gossypol, but they all have their drawbacks. Recently, high performance liquid chromatography has become increasingly widely used. However, due to more phenolic hydroxyl groups in gossypol, it is difficult to find the ideal chromatographic conditions. Abou-Donia et al. reported that on a Bondacash C 18 column with an octadecyl bonding phase, 0.1% phosphoric acid was used. % methanol for mobile phase separation to identify the purity of gossypol, Fong et al.