利用小球藻进行光合作用,使放射性~(14)CO_2参入到细胞体内,再从~(14)C标记的小球藻细胞体中提取蛋白质,经水解、分离、提纯和鉴定,得到十六种~(14)C-全标记氨基酸。产品为层析放化纯(纯度>99.5％),比放射性与法、意、比三国联营公司的同年产品相当。投入原料B_a~(14)CO_399mCi,得21.2mCi~(14)C-全标记氨基酸,利用第一次培养小球藻的废液再培养又获得11.5mCi~(14)C-小球藻蛋白质水解液,两者产率32.7％。 Using Chlorella for photosynthesis, the radioactive 14 CO2 was incorporated into the cell body, and proteins were extracted from the cell bodies labeled with ~(14)C. After hydrolysis, separation, purification, and identification, 16 were obtained. A ~(14)C-full-labeled amino acid. The product was purely chromatographic (purity >99.5%), which was comparable to radioactivity and France, Italy, and the three countries’ associate companies in the same year. The raw material B_a~(14)CO_399mCi was used to obtain a 21.2mCi~(14)C-full-labeled amino acid, and 11.5 mCi~(14)C-chlorella protein hydrolyzed was obtained by reculturing the first cultured chlorella waste liquid. Liquid, both yield 32.7%.