庆大霉素被广泛应用于临床,庆大霉素对肾毒的作用已有不少报道,但对免疫的影响却很少报道。本文着重观察了庆大霉素对小鼠的免疫器官、血液的ANAE(+)细胞数和体液免疫反应的影响,为临床在考虑大剂量、较长时间使用庆大霉素时提供免疫学的参考资料。方法 1.实验一:选用8周龄雄性、瑞士种小鼠56只,随机分成两组。实验组小鼠,每天腹腔注射庆大霉素100mg/Kg体重,连续17天,对照组小鼠每天注等量的生理盐水,动物于注射后的第7天、第17天取血并处死,检测外周血中ANAEE(+)(酸性醋酸萘酯酶活性)淋巴细胞的百分数。称胸腺和脾脏重量,并作组织切片,作H.E和甲基绿哌咯宁染色。此外还检测了血尿素氮、血肌酐的含量和肾脏的形态学变化 Gentamicin is widely used in clinical practice. There have been many reports on the effects of gentamycin on nephrotoxicity, but the impact on immunity has rarely been reported. This article focuses on the effects of gentamycin on the number of ANAE(+) cells and humoral immune responses in the immune organs and blood of mice, providing immunology for clinical considerations of large doses and longer use of gentamicin. Reference materials. Methods 1. Experiment 1: 56 male and female Swiss mice aged 8 weeks were randomly divided into two groups. The mice in the experimental group were intraperitoneally injected with gentamycin 100 mg/Kg body weight daily for 17 consecutive days. The mice in the control group received equal daily doses of normal saline. The animals were bled and sacrificed on the 7th and 17th days after the injection. The percentage of ANAEE (+) (acid naphthyl acetatease activity) lymphocytes in peripheral blood was measured. Thymus and spleen weights were weighed and histological sections were made for staining with H.E and methyl green peneberrin. In addition, blood urea nitrogen, serum creatinine levels, and morphological changes in the kidneys were also measured.