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本研究探讨了不同水体盐度对弓斑东方鲀α-淀粉酶、胃蛋白酶和胰蛋白酶活性的影响,同时得出最适于该鱼种消化吸收养分的盐度值,以发挥其生长潜力。试验用360尾鱼,分为4个处理(淡水对照,盐度8‰、18‰和35‰),每处理3个重复,每个重复30尾,通过15 d饲养试验,采样时间分别为:第1天、第4天、第7天和第15天。结果显示:随着盐度的增加,α-淀粉酶、胃蛋白酶和胰蛋白酶的活性有显著变化(P<0.05)。在试验后期,α-淀粉酶活性在盐度18‰时显著高于盐度8‰和35‰的情况(P<0.05);胃蛋白酶活性则在8‰和18‰2种盐度下有显著差异(P<0.05);胰蛋白酶活性在8‰时最大,显著高于35‰和18‰处理(P<0.05)。试验结果表明弓斑东方鲀饲养的水体盐度为8‰~18‰时能更有效的促进其消化酶的活性。
In this study, the effects of different salinities on the activities of α-amylase, pepsin and trypsin were studied. At the same time, the salinity values which were most suitable for the digestion and nutrient absorption of this species were obtained to exert their growth potential. The experimental fish were divided into four treatments (freshwater control, salinity 8 ‰, 18 ‰ and 35 ‰). Each treatment consisted of three replicates, each of which had 30 replicates. After 15 days of feeding, the sampling times were: Day 1, Day 4, Day 7 and Day 15. The results showed that the activities of α-amylase, pepsin and trypsin changed significantly with the increase of salinity (P <0.05). At the end of the experiment, α-amylase activity was significantly higher at salinity 18 ‰ than at 8 ‰ and 35 ‰ (P <0.05); pepsin activity was significant at 8 ‰ and 18 ‰ salinity (P <0.05). The trypsin activity was the highest at 8 ‰, which was significantly higher than that of 35 ‰ and 18 ‰ (P <0.05). The results showed that when the salinity of the water fed by Bunting catfish was 8 ‰ ~ 18 ‰, the activity of digestive enzymes was more effectively promoted.