论文部分内容阅读
目的探讨P-5m八肽对肝癌HepG2细胞中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的影响,以及P-5m八肽的抗肿瘤作用机制.方法用终浓度为10μmol/L和100μmol/L的P-5m八肽分别对处于对数生长期的HepG2细胞进行处理,药物作用时间为36 h,36 h后将细胞裂解.采用Real-time PCR方法检测给药后细胞中MMP-2和MMP-9 m RNA表达水平的变化;Western blot方法测定MMP-2和MMP-9蛋白表达水平的变化.结果Real-time PCR检测显示终浓度为10μmol/L和100μmol/L的P-5m八肽对HepG2细胞刺激后,MMP-2和MMP-9的m RNA表达均受到抑制,与对照组比较差异具有统计学意义(P<0.05);Western blot检测结果表明:用10μmol/L和100μmol/L的P-5m八肽对HepG2细胞刺激后,MMP-2和MMP-9的蛋白表达均受到抑制,与对照组比较差异具有统计学意义(P<0.05).结论 P-5m八肽可明显抑制HepG2细胞中MMP-2和MMP-9的表达.
Objective To investigate the effect of P-5m octapeptide on the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in HepG2 cells and the antitumor mechanism of P-5m octapeptide.Methods HepG2 cells in logarithmic growth phase were treated with P-5m octapeptide at the final concentration of 10μmol / L and 100μmol / L, respectively, and the drug was treated for 36 hours, then the cells were lysed after 36 hours.Real-time PCR The expression of MMP-2 and MMP-9 mRNA in the cells was detected by Western blot, and the protein expression of MMP-2 and MMP-9 was detected by Western blot.Results Real-time PCR showed that the final concentration was 10μmol / L And 100μmol / L P-5m octapeptide on HepG2 cells inhibited the mRNA expression of MMP-2 and MMP-9, the difference was statistically significant compared with the control group (P <0.05); Western blot results The results showed that the protein expressions of MMP-2 and MMP-9 were inhibited by HepG2 cells stimulated with 10μmol / L and 100μmol / L of P-5m octapeptide compared with the control group (P <0.05) .Conclusion The P-5m octapeptide can obviously inhibit the expression of MMP-2 and MMP-9 in HepG2 cells.