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目的:研究高氧环境下N-乙酰半胱氨酸(NAC)和牛磺酸(Tau)对离体兔晶状体的作用。方法:新鲜兔晶状体24只随机分为对照组、高氧组、20mmol/L NAC处理组和80mmol/L Tau处理组。除对照组正常培养外,其余各组培养在高氧环境(氧浓度>80%)下4h/d×7。每天观察其透明度,培养7d后检测各项生化指标。结果:培养7d后高氧组晶状体出现重度皮质混浊,正常组和80mmol/L牛磺酸处理组中度皮质混浊,20mmol/L NAC处理组仅轻度皮质混浊;高氧组与对照组相比谷胱甘肽含量及Na, K-ATPase活性分别下降了37.8%(P<0.05)和53.5%(P<0.05),两药物处理组均有不同程度升高,20mmol/L NAC处理组较80mmol/L Tau处理组升高更明显;过氧化氢酶、谷胱甘肽还原酶活性及水溶性蛋白含量的变化在各组间没有统计学意义。结论:NAC对高氧环境下兔晶状体皮质混浊的抑制作用优于Tau。
Objective: To study the effect of NAC and Tau on isolated rabbit lens in hyperoxia. Methods: Twenty - four fresh rabbit lenses were randomly divided into control group, hyperoxia group, 20mmol / L NAC group and 80mmol / L Tau group. Except normal control group, all the other groups were cultured in high oxygen environment (oxygen concentration> 80%) for 4h / d × 7. Observe the transparency of each day, after testing 7d biochemical indicators. Results: After 7 days of culture, severe cortical opacity occurred in the hyperoxia group and moderate cortical turbidity in the normal and 80 mmol / L taurine groups. Only mild cortical opacity was observed in the 20 mmol / L NAC group. Compared with the control group Glutathione content and Na, K-ATPase activity decreased by 37.8% (P <0.05) and 53.5% (P <0.05), respectively. / L Tau treatment group increased more obvious; catalase, glutathione reductase activity and water-soluble protein content changes in each group was not statistically significant. CONCLUSION: NAC is superior to Tau in the inhibition of cortical opacity in rabbit lens under hyperoxia.