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目的探讨血小板介导的ADCC效应在旋毛虫病免疫中的作用。方法采用雄性Wistar大鼠为旋毛虫感染的动物模型,将大鼠随即分为7组,观察免疫血清(原血清、经56℃30 min灭活补体和56℃4 h同时灭活补体及IgE血清)存在时血小板介导的杀伤旋毛虫肌幼虫作用。结果 1)正常对照组大鼠血清总IgE为(132.53±12.45)mg/L。感染组大鼠感染旋毛虫后1周,血清总IgE水平开始升高,为(202.70±11.60)mg/L,第3周达高峰,为(275.93±14.44)mg/L,与对照组比较差异有统计学意义(P<0.05);2)感染组大鼠血清特异性IgE阳性率以第2周和3周为最高(均为88.89%),与对照组(11.11%)相比差异有统计学意义(χ2=0.003,P<0.05)。3)感染鼠和正常鼠血小板均能介导对旋毛虫幼虫的杀伤作用,以48 h时的作用更强。结论血小板介导的ADCC参与了大鼠旋毛虫病早期的抗虫免疫;无论是感染鼠还是非感染鼠血小板介导的ADCC均有杀伤旋毛虫肌幼虫的作用,但以感染鼠血小板介导的ADCC作用更强。
Objective To investigate the role of platelet-mediated ADCC in trichinosis immunity. Methods The male Wistar rats were used as the animal model of Trichinella spiralis infection. The rats were randomly divided into 7 groups. The immune serum (original serum, inactivated serum at 56 ℃ for 30 min and inactivated at 4 ℃ for 56 h, complement and IgE serum ) Platelet-mediated killing of Trichinella spiralis larvae in the presence of. Results 1) The serum IgE of the normal control group was (132.53 ± 12.45) mg / L. The level of total serum IgE began to increase (202.70 ± 11.60) mg / L and peaked at the third week (275.93 ± 14.44) mg / L in infected group 1 week after infection with Trichinella spiralis, which was significantly lower than that of the control group (P <0.05); 2) The positive rate of serum specific IgE in the infected group was the highest at the second week and the third week (both 88.89%), which was statistically different from the control group (11.11%) Significance (χ2 = 0.003, P <0.05). 3) Both infected and normal rat platelets could mediate the killing effect on Trichinella spiralis larvae, and the effect was stronger at 48 h. Conclusion Platelet-mediated ADCC is involved in the early stage of anti-worm immunity in rat trichinosis. Platelet-mediated ADCC in both infected and non-infected mice has the effect of killing Trichinella spiralis muscle larvae. However, ADCC stronger role.