组建了一个仅含ＰＲ 启动子的原核高效表达载体ｐＲＣ， 它同时含有ｃⅠ调控基因、多酶切位点和两个强的转录终止序列． 现已成功地用于表达重组人肿瘤坏死因子α（ｈＴＮＦα）、重组人白细胞介素３ （ｈＩＬ３） 和抗溶菌酶（ＨＥＬ） 抗体Ｆｄ 基因， 表达量均占菌体总蛋白的３６％ 以上． 同时还研究了不同的宿主菌和原核增强子序列等因素对ＰＲ 启动子载体表达的影响． 此外， 还比较了分别以ＰＲ、ＰＬ 或ＰＲＰＬ 作为启动子时表达ｈＴＮＦα的情况，结果表明， 单用ＰＲ 或ＰＬ 启动子可获得与使用ＰＲＰＬ 串联启动子一样的高效表达 A prokaryotic expression vector pRC containing only the PR promoter was constructed, which contains both the c Ⅰ regulatory gene, multiple restriction sites and two strong transcription termination sequences. It has been successfully used to express recombinant human tumor necrosis factor  α (hTNF  α), recombinant human interleukin  3 (hIL  3) and anti-lysozyme (HEL) antibody Fd gene expression in both total bacterial cells More than 36% of the protein. The effects of different host bacteria and prokaryotic enhancer sequences on the expression of PR promoter were also studied. In addition, the expression of hTNF-α was also compared with PR, PL or PRPL as promoters respectively. The results showed that the expression of hTNF-α was the same as PRPL tandem promoter