以“雪峰”为转基因材料 ,建立了以子叶为外殖体 ,直接分化成苗的离体再生体系 ,其各阶段的培养基分别为 :(1 )芽分化培养基 :MS +BA 3～ 5mg/L +IAA 2mg/L ;(2 )芽伸长培养基 :MS +BA 1～ 2mg/L ;(3)生根培养基 :1 /2MS +IAA 0 .5～ 1mg/L。利用农杆菌介导的三亲交配法将抗菌肽基因CecropinB ,CecropinD导入辣椒 ,获得了再生植株 ,经PCR和Southern杂交检测 ,表明目的基因已整合到辣椒核基因组中 ,获得了转基因植株 Taking “Xuefeng” as the transgenic material, an in vitro regeneration system was established that cotyledon was used as explants and differentiated into seedlings directly. The culture media of each stage were as follows: (1) bud differentiation medium: MS + BA 3 ~ 5mg / L + IAA 2mg / L; (2) bud elongation medium: MS + BA 1 ~ 2mg / L; (3) rooting medium: 1 / 2MS + IAA 0.5 ~ 1mg / L. The antibacterial peptide genes CecropinB and CecropinD were introduced into pepper by Agrobacterium-mediated mating method and the regenerated plants were obtained. PCR and Southern blotting showed that the target gene had been integrated into the nuclear genome of pepper and the transgenic plants