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应用免疫组化（ＳＰ法）和革兰氏染色技术，对３６例胆囊腺癌和３０例慢性胆囊炎进行ｐ２１、ｐ５３蛋白及细菌Ｌ型检测，同时对Ｌ型阳性和阴性组织的ｐ２１、ｐ５３表达进行对比分析。结果发现，腺癌和慢性炎的革兰染色Ｌ型检出率分别为８０．６％和７６．７％，与Ｌ型抗原检出率均无显著性差异（Ｐ＞０．０５）。腺癌组的ｐ２１、ｐ５３表达率明显高于炎症组（Ｐ＜０．００５），腺癌中Ｌ型阳性组的ｐ２１、ｐ５３表达率也高于其Ｌ型阴性组（Ｐ＜０．０５）。表明Ｌ型感染与胆囊腺癌、慢性炎及ｐ２１、ｐ５３过表达有关，ｐ２１、ｐ５３过表达与胆囊腺癌也有关。提示Ｌ型参与了ｒａｓ、ｐ５３基因突变，并可能通过致基因突变而成为胆囊腺癌发生的危险因素之一。
Using immunohistochemistry (SP method) and Gram stain technique, p21, p53 protein and bacterial L-type were detected in 36 cases of gallbladder adenocarcinoma and 30 cases of chronic cholecystitis, and p21 and p53 were detected in L-positive and negative tissues. Expression for comparative analysis. The results showed that the detection rate of Gram-negative L-type adenocarcinoma and chronic inflammation were 80.6% and 76.7%, respectively, and there was no significant difference with the detection rate of L-type antigen (P>0.05). The expression rates of p21 and p53 in the adenocarcinoma group were significantly higher than those in the inflammatory group (P<0.005), and the expression rates of p21 and p53 in the adenocarcinoma of the L-type positive group were also higher than those in the L-negative group (P<0.05). . It showed that L-infection was associated with gallbladder adenocarcinoma, chronic inflammation, and overexpression of p21 and p53. Overexpression of p21 and p53 was also associated with gallbladder adenocarcinoma. It suggests that L-type is involved in the mutation of ras and p53 genes, and may become one of the risk factors of gallbladder adenocarcinoma through gene mutation.