目的 :制备用于检测转胸腺素基因蓝藻表达产物的特异性抗体。方法 :用提纯的小肽 (2 8个氨基酸 )胸腺素α1与牛血清白蛋白偶联后作为抗原 ,采用皮下多点注射免疫的方法免疫大鼠。经过 3个月的免疫 ,获得抗Tα的多克隆抗体。结果 :用间接酶联免疫吸附 (ELISA)方法 ,测得抗体效价超过 40 96。蛋白印迹 (Westernblot)检测结果显示 ,该抗体能特异性地与胸腺素α1抗原产生明显免疫亲和反应。结论 :所制备的抗体具有很好的灵敏性和特异 Objective: To prepare specific antibodies for detecting the expression products of the thymosin gene. METHODS: Purified small peptide (28 amino acids) thymosin α1 was conjugated with bovine serum albumin, then used as antigen to immunize rats by multi-point subcutaneous immunization. After 3 months of immunization, anti-Tα polyclonal antibodies were obtained. Results: The antibody titer was over 40 96 measured by indirect enzyme-linked immunosorbent assay (ELISA). The result of Western blot showed that the antibody could specifically immunoreactive with thymosin α1 antigen. Conclusion: The prepared antibody has good sensitivity and specificity