目的了解南京地区耐亚胺培南肠杆菌科细菌碳青霉烯酶(KPC酶)及整合子分布。方法收集南京地区3所三级甲等医院共55株耐亚胺培南肠杆菌科细菌,进行改良Hodge试验,PCR扩增检测细菌KPC酶与整合子基因并行序列分析。结果 55株待检菌中,改良Hodge试验阳性39株,阳性率为70.9%;KPC酶PCR扩增阳性39株,阳性率为70.9%;整合子PCR扩增阳性19株,阳性率为34.5%。结论 KPC酶引起的碳青霉烯类耐药需引起临床及实验室的关注。 Objective To understand the bacterial resistance to carbapenemase (KPC enzyme) and integron in Enterobacteriaceae in Nanjing area. Methods A total of 55 strains of Enterobacteriaceae resistant to imipenem were collected from three Grade A hospitals in Nanjing. The modified Hodge test was used to detect the sequence of bacterial KPC and integron genes by PCR. Results Among the 55 tested bacteria, 39 were positive with Modified Hodge test, the positive rate was 70.9%. The positive rate was 70.9% by PCR amplification of KPC enzyme. The positive rate of integrin PCR amplification was 19 with the positive rate of 34.5% . Conclusion Carbapenem resistance induced by KPC enzyme needs clinical and laboratory attention.