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In this manuscript, we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction (A-PCR) and loop-mediated isothermal amplification (LAMP) technology. Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions. We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection. The established method fished a fast, specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.