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为探讨结肠癌缺失基因（ＤＣＣ）与肺癌发病的关系，利用半定量的逆转录聚合酶链反应（ＲＴ－ＰＣＲ）结合Ｓｏｕｔｈｅｒｎ杂交分析了７例肺癌患者（４例鳞癌和３例腺癌）癌组织及正常肺组织中ＤＣＣ基因ｍＲＮＡ的表达，发现４例肿瘤组织中ＤＣＣ基因表达明显低于对照肺组织。７例标本分析结果表明肺癌组织中ＤＣＣ基因的表达水平为正常肺组织的４５％左右。对上述７例及另外８例标本的两个多态位点进行ＰＣＲ杂合性丢失（ＬＯＨ）分析，信息性个体占９例，其中５例表现杂合性丢失：２例在ＭｓｐＩ限制性长度多态片段（ＲＦＬＦ），３例为可变数量串连重复序列（ＶＮＴＲ）。本结果证明在肺癌组织中存在ＤＣＣ基因表达低下及ＬＯＨ改变，提示其可能为肺癌发病的易感基因之一。
To investigate the relationship between colon cancer deficient gene (DCC) and lung cancer, 7 lung cancer patients (4 squamous cell carcinomas and 3 adenocarcinomas) were analyzed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) combined with Southern blotting. DCC gene mRNA expression in cancer tissue and normal lung tissue found that DCC gene expression was significantly lower in tumor tissue than in control lung tissue in 4 cases. The analysis results of 7 specimens showed that the expression of DCC gene in lung cancer tissue was about 45% of normal lung tissue. Loss of heterozygosity (LOH) was analyzed in the two polymorphic loci in the above 7 cases and 8 other specimens. Informational individuals accounted for 9 cases, of which 5 cases showed loss of heterozygosity: 2 cases in MspI restrictive length Polymorphic fragment (RFLF), 3 cases of variable number of tandem repeats (VNTR). This result demonstrates that there is a low DCC gene expression and LOH changes in lung cancer tissues, suggesting that it may be one of the susceptibility genes for lung cancer.