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为了探讨旋转刺激与运动病发生的关系,本研究利用一种复杂的围绕两轴旋转的加速度刺激器刺激大鼠后,观察大鼠全脑内Fos蛋白的表达情况。动物被随机地分成四组,即正常对照组、两轴旋转刺激组、双侧迷路毁损组以及双侧迷路毁损后接受旋转刺激组。采用免疫组织化学染色方法观察全脑不同核团内Fos蛋白的表达情况。结果显示:(1)正常对照组和双侧迷路毁损组大鼠的脑内均未检测到Fos样免疫阳性产物;(2)两轴旋转刺激组的大鼠在给予复杂的围绕两轴旋转的加速度刺激后,在大鼠脑和脑干的多个核团内均可检测到Fos样免疫阳性神经元,其阳性产物主要表达于细胞核。其中在脑干内的前庭复合体的不同亚核(包括前庭内侧核、前庭上核和前庭下核),孤束核、蓝斑核、臂旁内侧核、臂旁外侧核,间脑的室旁核以及边缘系统的杏仁核等内均可观察到密集分布的Fos样免疫阳性神经元;(3)双侧迷路毁损组大鼠在接受复杂的围绕两轴旋转刺激后,在上述相应核团内均难以检测到Fos蛋白的表达。以上研究结果提示两轴旋转刺激可以有效地激活前庭神经元,而大鼠在接受前庭刺激后,脑和脑干的许多核团内大量的神经元可能通过与前庭核复合体发生直接或间接的纤维联系也被激活,这些被激活的神经元可能与运动病发生的复杂机制有关。
In order to investigate the relationship between rotational stimulation and motion sickness, the present study investigated the expression of Fos protein in whole brain of rats using a complex acceleration stimulator around two axes of rotation. The animals were randomly divided into four groups: normal control group, biaxial rotation stimulation group, bilateral labyrinthine lesion group, and bilateral labyrinthine lesion group, and received rotational stimulation group. Immunohistochemical staining was used to observe the expression of Fos protein in different nuclei of whole brain. The results showed that: (1) Fos-like immunopositive products were not detected in the brain of both normal control group and bilateral labyrinthine dystrophic rats; (2) After acceleration stimulation, Fos-like immunopositive neurons were detected in multiple nuclei of rat brain and brain stem, and the positive products were mainly expressed in the nucleus. Among them, different sub-nuclei (including medial vestibular nucleus, vestibular nucleus and vestibular nucleus), nucleus tractus solitarius, locus coeruleus, medial parabrachial nucleus, lateral parabrachial nucleus, (3) Fus-like immunoreactive neurons were observed in the paranasal nucleus, para-nucleus and apical amygdala of the limbic system. (3) After bilateral labyrinthine lesions were subjected to complex rotation around two axes, Fos protein expression was difficult to detect within. These results suggest that two-axis rotational stimulation can effectively activate the vestibular neurons, and rats in the vestibular stimulation, the brain and brain stem many nuclei within a large number of neurons may be through the vestibular nucleus complex occurs directly or indirectly Fiber connections are also activated, and these activated neurons may be involved in the complex mechanisms by which motion sickness occurs.