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旨在研究一种较好的微波和长春新碱的联合方法，用于白血病细胞的体外净化．将微波和长春新碱分别单独以及联合作用于正常骨髓细胞ＨＬ６０、Ｕ９３７和Ｋ５６２细胞株，通过ＧＭ－ＣＦＵ和ｔＣＦＵ的测定来评价体外净化的效果．结果发现微波和长春新碱对ＧＭ－ＣＦＵ、ｔＣＦＵ存活率均表现出抑制作用，抑制程度呈现出功率相关性和浓度相关性；在一定范围内，微波和长春新碱对３种细胞株ｔＣＦＵ存活率的抑制显著大于ＧＭ－ＣＦＵ（Ｐ＜０．０５或Ｐ＜０．０１）；微波和长春新碱联合作用对３种细胞株的杀伤作用比单用长春新碱有显著性增加（Ｐ＜０．０５），而不显著增加对ＧＭ－ＣＦＵ的抑制（Ｐ＞０．０５）．这一结果提示微波和长春新碱联合用于白血病细胞的体外净化能产生协同效应，值得进一步研究．
3. The aim is to study a better combination of microwave and vincristine for in vitro purification of leukemia cells. The microwave and vincristine were used separately and in cooperation with normal bone marrow cells HL60, U937 and K562 cell lines. The in vitro purification effect was evaluated by GM-CFU and tCFU. The results showed that microwave and vincristine showed inhibitory effects on the survival rate of GM-CFU and tCFU, and the degree of inhibition showed a correlation between power and concentration. Within a certain range, microwave and vincristine survived on the three cell lines tCFU. The inhibition of the rate was significantly greater than that of GM-CFU (P<0.05 or P<0.01). The combined effect of microwave and vincristine on the killing of the three cell lines was significantly greater than that of vincristine alone (P< 0.05), but not significantly increased the inhibition of GM-CFU (P> 0.05). This result suggests that the combined use of microwave and vincristine in the in vitro purification of leukemia cells can produce a synergistic effect and is worth further investigation.