目的研究单增李斯特氏菌核酸层析检测试剂盒的准确性和特异性。方法从市场采集自然样品70份,经过2步增菌后,用核酸层析检测试剂盒进行检测,同时用传统方法进行验证。结果 70份样品试剂盒检测阳性3份,阴性67份,而传统方法检测阳性4份,其中1份阳性样品试剂盒检测为阴性。试剂盒检测的准确率达到98.60%。特异性试验表明该剂盒检测标准菌株特异性好,与常见的食源性致病菌均无交叉反应。结论单增李斯特氏菌核酸层析检测层析试剂盒特异性好,检测准确率高,能够满足食品中单增李斯特氏菌检测的需要。 Objective To study the accuracy and specificity of Listeria monocytogenes detection kit. Methods 70 samples of natural samples were collected from the market. After 2 steps of enrichment, the samples were detected by using the nucleic acid chromatography detection kit, and the traditional methods were used for verification. Results 70 samples were tested positive 3, negative 67, while the traditional method of detection of 4 positive, of which 1 positive sample kit was negative. Kit detection accuracy of 98.60%. Specific tests showed that the kit test standard strains of good specificity, and common food-borne pathogens no cross-reaction. Conclusion Listeria monocytogenes detection chromatography kit has good specificity and high detection accuracy, which can meet the need of Listeria monocytogenes detection in food.