论文部分内容阅读
目的了解五华县手足口病病原学特征及变化规律,为科学制定防控策略提供可靠依据。方法采集2010-2012年五华县哨点监测医院诊治的普通手足口病病例的粪便标本,使用实时荧光定量PCR法检测总肠道病毒(EV)、EV 71和CoxA16核酸。结果共采集152例病例粪便标本,EV阳性115例,阳性率75.66%,不同年份EV阳性率差异无统计学意义(P>0.05);EV71、CoxA16和其他肠道病毒阳性率分别为23.03%、18.42%和34.21%,差异有统计学意义(P<0.01)。2010年流行优势株为EV71,2011～2012年流行优势株为其他肠道病毒,不同性别、不同年龄EV71、CoxA16和其他肠道病毒阳性率差异均无统计学意义(P>0.05)。EV71、CoxA16和其他肠道病毒三种型别的肠道病毒全年交替变更流行。结论五华县手足口病流行优势株发生动态改变。加强手足口病病原学监测研究,掌握毒株变化规律,有助于更好地采取预防和控制措施。
Objective To understand the etiological characteristics and changes of hand-foot-mouth disease in Wuhua County and provide a reliable basis for making scientific prevention and control strategies. Methods Stool specimens from common HFMD cases diagnosed and treated in Wuhua County sentinel surveillance hospital from 2010 to 2012 were collected. Total enterovirus (EV), EV71 and CoxA16 nucleic acids were detected by real-time fluorescence quantitative PCR. Results A total of 152 cases of stool samples were collected and the positive rate of EV positive was 75.66%. There was no significant difference in EV positive rate in different years (P> 0.05). The positive rates of EV71, CoxA16 and other enterovirus were 23.03% 18.42% and 34.21%, the difference was statistically significant (P <0.01). The predominant strain was EV71 in 2010, and the predominant strains were other enteroviruses in 2011-11. There was no significant difference in the positive rates of EV71, CoxA16 and other enterovirus among different sexes, different ages (P> 0.05). EV71, CoxA16 and other enteroviruses of three types of enterovirus alternating prevalence throughout the year. Conclusion The prevalence of HFMD in Wuhua County is changing dynamically. To strengthen the hand foot and mouth disease etiology monitoring research, master the variation of strains, help to better take prevention and control measures.