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本实验主要分析青蒿素作用后p53功能突变的人乳腺癌细胞MDA-MB-435微核的变化。通过MTT法测定不同药物浓度及不同药物作用时间对细胞毒性的影响,通过胞质分裂阻滞法(CB法)计数青蒿素作用联合不同剂量60Coγ射线照射后细胞的微核率(Micronucleus frequency,MNF)和微核细胞率(Micronucleus cell frequency,MNCF),将青蒿素作用的细胞与单纯照射细胞的微核率和微核细胞率的统计分析结果进行比较。实验结果表明,青蒿素对实验细胞MDA-MB-435毒性较小,在实验药物浓度为200μmol/L,作用时间为24h时,将CB微核法得到的数据进行回归拟合可以得到药物作用及未经药物作用的剂量效应曲线,比较两组曲线,青蒿素作用组的微核率及微核细胞率明显高于单纯照射细胞(p>0.05)。
This experiment mainly analyzed the change of micronucleus of human breast cancer cell line MDA-MB-435 with p53 mutation after artemisinin treatment. MTT assay was used to determine the effect of different drug concentrations and time of different drugs on cytotoxicity. The micronucleus frequency of cells after artemisinin combined with different doses of 60Coγ-rays was counted by cytokinin-block method (CB method) MNF and Micronucleus Cell Frequency (MNCF) were used to compare the results of statistical analysis of artemisinin-treated cells with the micronuclei and micronuclei rates of irradiated cells. The experimental results showed that artemisinin was less toxic to MDA-MB-435 cells. When the experimental drug concentration was 200 μmol / L and the action time was 24 h, the data obtained by CB micronucleus method were fitted by regression to obtain the drug effect And dose-response curve without drug effect. Comparing the two curves, the micronucleus rate and micronucleus rate of artemisinin-treated group were significantly higher than those of pure irradiation group (p> 0.05).