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目的:评价青光眼滤过手术中应用陈旧生物羊膜抗纤维增殖作用。方法:采取随机对照动物实验研究方法,选日本大耳兔30只,共60眼,采用随机数字表法将其分为两组。两组中右眼(30眼)均行小梁切除术,术中巩膜瓣层间植入陈旧生物羊膜,组A左眼(15眼)行小梁切除术联合应用干扰素α-2b,组B左眼(15眼)仅行单纯小梁切除术。并于术后3,7,14,30,60d分别处死两组中各3只动物,摘除眼球作病理切片,观察羊膜降解时间、成纤维细胞和炎症细胞计数。结果:羊膜组早期即可见大量成纤维细胞增殖,成纤维细胞处于功能活跃状态。羊膜组炎性细胞数目、成纤维细胞数目,术后3,7,14d均高于其它两组。羊膜大致在14～21d内降解。结论:兔眼小梁切除术中应用陈旧的生物羊膜诱发了兔眼的免疫排斥反应。
Objective: To evaluate the anti-proliferative effects of old biological amnion in glaucoma filtration surgery. Methods: A randomized controlled animal experimental study method, 30 Japanese big-eared rabbits were selected, a total of 60 eyes, using random number table method is divided into two groups. In both groups, the right eye (30 eyes) underwent trabeculectomy. The scleral flap was implanted into the amniotic membrane in the right eye. The left eye (15 eyes) in group A underwent trabeculectomy combined with interferon α-2b B left eye (15 eyes) only simple trabeculectomy. Three rabbits in each group were killed at 3, 7, 14, 30, and 60 days after operation. The eyes were removed for histopathological examination. The amniotic membrane degradation time, fibroblast and inflammatory cell count were observed. Results: A large number of fibroblasts proliferated and the fibroblasts were active in the early stage of amniotic membrane. The number of inflammatory cells and the number of fibroblasts in amniotic membrane group were higher than the other two groups at 3, 7 and 14 days after operation. Amniotic membrane degradation in about 14 ~ 21d. Conclusion: The application of old biological amniotic membrane in rabbit trabeculectomy induced immune rejection in rabbit eyes.