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采用逆转录－ＰＣＲ的方法从骨髓基质细胞中扩增出人干细胞因子基因，并与载体ｐＫＫ２３３－２和ｐＵＣ１８重组。经该基因的限制酶酶解图谱分析及ＤＮＡ序列分析证实。
Human stem cell factor genes were amplified from bone marrow stromal cells by reverse transcription-PCR and recombined with the vectors pKK233-2 and pUC18. The restriction endonuclease analysis of the gene and DNA sequence analysis confirmed.