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试验以西南地区两个优良玉米自交系R08与18-599R为材料,采用苗期人工接种玉米生平脐蠕孢菌(Bipolariszeicola)进行抗性鉴定,并对接种后自交系叶片中的活性氧(ROS)、抗氧化物酶类(SOD,POD,CAT)活性、丙二醛(MDA)质量分数、抗病相关基因(PR1,ZmDREB2A和ZmERF3)表达及过敏性反应(HR)等抗病相关反应进行了检测,为玉米抗病育种提供依据.结果表明:1 R08对B.zeicola表现感病,18-599R为高感;2叶片受侵染后,ROS在R08中迅速且大量地积累,而在18-599R相对滞后且量少;3 POD,SOD,CAT活性随接种时间呈上升趋势,在接种后12~24h左右出现峰值,随后降低,但其增幅存在差异,同时,MDA质量分数在R08中比18-599R更高;43个基因于接种早期在18-599R中微量表达后均被抑制,而R08在12~24h大量表达,随后降低;5 R08叶片在接种后出现严重的HR反应,而18-599R相对较轻.
In this study, two elite maize inbred lines R08 and 18-599R in southwestern China were used as materials to evaluate the resistance of maize Bipolariszeicola inoculated at seedling stage. The effects of reactive oxygen species (ROS), antioxidant enzymes (SOD, POD, CAT) activity, MDA content, PR1, ZmDREB2A and ZmERF3 expression and allergic reaction (HR) The results showed that: 1 R08 was susceptible to B. zeicola and 18-599R was highly susceptible; ROS was rapidly and massively accumulated in R08 after infected with 2 leaves, While the activity of POD, SOD and CAT increased with the inoculation time, and peaked at 12 ~ 24h after inoculation, and then decreased, but the difference was significant. Meanwhile, the content of MDA R08 was higher than that of 18-599R; 43 genes were inhibited after micro-expression in 18-599R in the early stage of inoculation, while R08 was highly expressed in 12 ~ 24h, then decreased; 5R08 leaves severe HR reaction after inoculation , While 18-599R is relatively light.